Table 2.
Summary table of the results of the confirmatory mutation test.
| Confirmatory mutation test (preincubation test)∗ | ||||||||||||||||||||
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| Concentrations (μg/plate) | Salmonella typhimurium tester strains | Escherichia coli | ||||||||||||||||||
| TA 98 | TA 100 | TA 1535 | TA 1537 | WP2uvrA | ||||||||||||||||
| -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | |||||||||||
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| Mean values of revertants per plate and mutation rate (MR) | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR |
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| Untreated control | 14.7 | 0.96 | 22.7 | 1.33 | 102.0 | 1.02 | 126.3 | 1.10 | 8.7 | 0.93 | 8.0 | 0.83 | 9.3 | 1.27 | 9.0 | 0.84 | 13.3 | 0.77 | 19.3 | 1.16 |
| DMSO control | 15.3 | 1.00 | 17.0 | 1.00 | 100.0 | 1.00 | 114.3 | 1.00 | 9.3 | 1.00 | 9.7 | 1.00 | 7.3 | 1.00 | 10.7 | 1.00 | 17.3 | 1.00 | 16.7 | 1.00 |
| Ultrapure water control | – | – | – | – | 86.7 | 1.00 | – | – | 13.0 | 1.00 | – | – | – | – | – | – | 17.7 | 1.00 | – | – |
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| 5000 | 5.0 | 0.33 | 16.7 | 0.98 | 44.3 | 0.44 | 61.3 | 0.54 | 0.3 | 0.04 | 9.0 | 0.93 | 0.7 | 0.09 | 4.7 | 0.44 | 12.7 | 0.73 | 16.3 | 0.98 |
| 1600 | 9.0 | 0.59 | 23.3 | 1.37 | 46.7 | 0.47 | 71.3 | 0.62 | 2.7 | 0.29 | 8.3 | 0.86 | 2.0 | 0.27 | 8.7 | 0.81 | 16.3 | 0.94 | 17.7 | 1.06 |
| 500 | 8.3 | 0.54 | 18.0 | 1.06 | 45.7 | 0.46 | 69.7 | 0.61 | 6.0 | 0.64 | 9.3 | 0.97 | 0.7 | 0.09 | 8.3 | 0.78 | 14.3 | 0.83 | 15.3 | 0.92 |
| 160 | 12.3 | 0.80 | 26.7 | 1.57 | 80.3 | 0.80 | 91.0 | 0.80 | 8.3 | 0.89 | 10.0 | 1.03 | 4.3 | 0.59 | 9.3 | 0.88 | 10.7 | 0.62 | 20.3 | 1.22 |
| 50 | 12.3 | 0.80 | 26.3 | 1.55 | 94.7 | 0.95 | 100.7 | 0.88 | 10.0 | 1.07 | 10.0 | 1.03 | 7.7 | 1.05 | 10.3 | 0.97 | 16.0 | 0.92 | 18.0 | 1.08 |
| 16 | 14.0 | 0.91 | 29.0 | 1.71 | 94.7 | 0.95 | 101.0 | 0.88 | 9.3 | 1.00 | 8.7 | 0.90 | 7.7 | 1.05 | 11.7 | 1.09 | 12.3 | 0.71 | 19.3 | 1.16 |
| 5 | 14.3 | 0.93 | 23.0 | 1.35 | 94.7 | 0.95 | 115.7 | 1.01 | 10.3 | 1.11 | 13.0 | 1.34 | 7.3 | 1.00 | 11.3 | 1.06 | 16.0 | 0.92 | 18.3 | 1.10 |
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| NPD (4) | 310.0 | 20.22 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SAZ (2) | – | – | – | – | 1389.3 | 16.03 | – | – | 3104.0 | 238.77 | – | – | – | – | – | – | – | – | – | – |
| 9AA (50) | – | – | – | – | – | – | – | – | – | – | – | – | 586.0 | 79.91 | – | – | – | – | – | – |
| MMS (2 μL) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 898.7 | 50.87 | – | – |
| 2AA (2) | – | – | 989.3 | 58.20 | – | – | 1752.0 | 15.32 | – | – | 113.3 | 11.72 | – | – | 133.7 | 12.53 | – | – | – | – |
| 2AA (50) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 181.3 | 10.88 |
∗DMSO was applied as vehicle of the test article and positive control substances: NPD, 9AA, and 2AA; and the ultrapure water was applied as vehicle for the SAZ and MMS. The mutation rate of the test item and the untreated control is given referring to the DMSO.