Figure 8.

Anti-Fg immunostaining of aortic sinuses from typical L^−/−/A^−/− mouse at various ages. (a) 24 weeks of age. Fg immunostains demonstrate that diffuse fibrin deposits (red arrowheads) are present in the subluminal spaces and at the base of the lesion above the media. (b) 36 weeks of age. Anti-Fg immunostains demonstrate variable deposits of fibrin within the subcapsular region (red arrowhead), as well as some focal, but faint, positive areas associated with the endothelium (blue arrowhead) and lipid core above the media (black arrowheads). (c) 48 weeks of age. Anti-Fg immunostaining demonstrates that the underlying subcapsular region is abundant in fibrin deposits (red staining) and patchy areas of fibrin are associated with the endothelium (blue arrowhead). Fibrin is also identified at breakpoints on the endothelium (black arrowhead). Diffuse areas of fibrin are evident at the base of the lipid core above the media (yellow arrowheads). (d) 60 weeks of age. Anti-Fg immunostaining demonstrates fibrin deposition (red staining) scattered throughout the subcapsular and core regions. Original magnification, 100X.