Figure 2.
Method performance overview. (A) Concentrations of individual isotopologues are calculated by deconvolution. Crosstalk between adjacent channels caused by natural 13C isotopic abundances and imperfect labeling of reagents. Blue: Calculated values from theoretic isotopic distribution and specified labeling extent of reagents. Green: Experimental values from pure standards. (B) Stability of derivatized analytes over time at 4 °C. (C) Stability of derivatized folates during vacuum concentration. (D) Matrix effects of E. coli lysate (purple) and HepG2 lysate (green) at two different spiked in standard concentrations (conc) compared to buffer (blue). *p < 0.01.