Fig. 4.

miR-146a determines neuronal lineage identities. Heatmap of gradient of expression from the Cell Cycle (a) and Neuronal (b) Modules spanning human fetal development to late adulthood in distinct brain regions. A1C, auditory cortex; AMY, amygdala; CBC, cerebellar cortex; DFC, dorsolateral prefrontal cortex; HIP, hippocampus; IPC, posterior inferior parietal cortex; ITC, inferior temporal cortex; M1C, primary motor cortex; MD, mediodorsal nucleus of thalamus; MFC, medial prefrontal cortex; OFC, orbital prefrontal cortex; S1C, primary somatosensory cortex; STC, superior temporal cortex; STR, striatum; V1C, primary visual cortex; VFC, ventrolateral prefrontal cortex. Period labels: 3—Early fetal (10 ≤ Age ≤ 13 Post conception week, PCW); 4—Early midfetal (13 ≤ Age ≤ 16 PCW); 5—Early midfetal (16 ≤ Age ≤ 19 PCW); 6—Late midfetal (19 ≤ Age ≤ 24 PCW); 7—Late fetal (24 ≤ Age ≤ 38 PCW); 8—Neonatal and early infancy (Birth ≤ Age ≤ 6 Postnatal months, M); 9—Late infancy (6 ≤ Age ≤ 12 M); 10—Early childhood (1 ≤ Age ≤ 6 Postnatal years, Y); 11—Middle and late childhood (6 ≤ Age ≤ 12 Y); 12—Adolescence (12 ≤ Age ≤ 20 Y); 13—Young adulthood (20 ≤ Age ≤ 40 Y); 14—Middle adulthood (40 ≤ Age ≤ 60 Y); 15—Late adulthood (60 Y ≤ Age). c Expression difference of detectable neuronal specific markers in differentiated H9 hNSC. Dotted lines indicate expression difference threshold (fold change ≥ 1.5). *P < 0.05, **P < 0.01 and ***P < 0.001 by EdgeR analysis (as representative of 3 different RNA-Seq analyses)