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. 2001 Oct 16;98(22):12671–12676. doi: 10.1073/pnas.231140698

Figure 5.

Figure 5

Hepatic UGT expression in indinavir-treated and untreated rats. Total RNA was isolated from the livers of untreated (−) heterozygous Gunn (j/+) and wild-type Wistar (+/+) rats and from animals treated with four doses of indinavir (240 mg/kg) every 8 h via gavage (+). Northern blotting (25 μg of RNA) was performed by using a murine cDNA probe specific for exon 1 of UGT1A1 (Upper Left) and a nonspecific UGT probe (Upper Right). (Lower) 500 μg of whole liver homogenate (Left) and 50 μg of isolated hepatic microsomes (Right) from indinavir-treated (+) and untreated (−) rats was subjected to Western blotting with rabbit antiserum (1:500 dilution).