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. 2018 Jun 20;16:172. doi: 10.1186/s12967-018-1545-0

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Evaluation of CD4⁺ T cells frequency in peripheral blood of CLL patients. Representative box plots relative to a CD4⁺ T cell subsets frequency in PBMCs from HV (n = 15) and CLL patients (n = 15); b IFN-γ, IL-4 and IL-17A secretion in CD4⁺ T cells obtained from HV (n = 15) and CLL patients (n = 15); c IFN-γ, IL-4, IL-17A co-secretion with IL-10 in CD4⁺ T cells obtained from HV (n = 15) and CLL patients (n = 15); all after in vitro stimulation with IL-6 and PIM. All results are expressed as median and interquartile range. P value shown is obtained from the comparison between the indicated groups by exact non-parametric Mann–Whitney U test (*P < 0.05; **P < 0.01; ***P < 0.001)