Table 2.
Verification of real-time PCR for some differentially expressed genes
| Genes | Transcriptomics fold change (infected/control) | qRT-PCR fold change (2−∆∆Ct) (infected/control) | Gene function |
|---|---|---|---|
| RIG-1 | 21.3 | 6.69 | RIG-like receptor |
| MDA5 | 37.3 | 31.48 | RIG-like receptor |
| TLR7 | 6.429 | 25.3 | Toll-like receptor |
| TLR3 | 4.564 | 4.23 | Toll-like receptor |
| IFN-α | Inf | 160.9 | Type I IFN |
| IFN-β | 226.29 | 702.86 | Type I IFN |
| IFN-γ | 7.66 | 5.63 | Type II IFN |
| IL-1β | – | 78.09 | Proinflammatory cytokines |
| IL-2 | Inf | 171.88 | Proinflammatory cytokines |
| IL-6 | 31.16 | 68.62 | Proinflammatory cytokines |
| MX | 1464 | 3880.75 | IFN-stimulated genes |
| OAS | 442.74 | 191.77 | IFN-stimulated genes |
| PKR | 31.25 | 19.85 | IFN-stimulated genes |
| IFIT5 | 4110.1 | 2103.33 | IFN-stimulated genes |
“Inf” represented the differential expression is infinite is in the results of transcriptome sequencing.
“–” represented the gene did not detect through RNAseq.