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. 2018 Jun 20;49:51. doi: 10.1186/s13567-018-0548-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Ovotransferrin stability in faeces from birds. The ovotransferrin stability in the faeces was assessed by spiking two independent faeces pools from healthy birds with ovotransferrin at a final concentration of 200 µg/g. A The spiked samples were aliquoted and incubated at room temperature for 0, 1, 2, 6 or 24 h after which the ovotransferrin concentration was measured by ELISA. B The ovotransferrin stability in the faeces with (black) or without (grey) the addition of protease inhibitors was assessed after 0, 2 or 24 h incubation at room temperature. The % ovotransferrin recovery was calculated relative to the 0 h timepoint.