Fig. 2.

LncGPR107 was highly expressed in liver cancer cells and TICs. a Total RNA from 19 peri-tumor, 7 early HCC and 12 advanced HCC was extracted and lncGPR107 expression was detected with realtime PCR. b The correlation between GPR107 and lncGPR107 expression levels in tumor samples were shown. c RNA sample from peri-tumor and tumor samples were used for lncGPR107 Northern blot, revealing the high expression of lncGPR107 along with liver tumorigenesis. d In situ hybridization of lncGPR107. LncGPR107^high cell ratios were shown in middle panels and lncGPR107 photon intensity was shown in right panels. e, f LncGPR107 expression levels in CD133⁺ liver TICs (e) and stem-like oncospheres (f) were examined through realtime PCR. g, h Fluorescence in situ hybridization (FISH) analysis for lncGPR107 expression and localization in TIC/non-TIC (g) or sphere/non-sphere (h). ZIC2 and CD133 were liver TIC markers. Scale bars, 10 μm. Experiments were repeated at least three times