Fig. 3.

LncGPR107 played an essential role in liver TIC self-renewal. a LncGPR107 were depleted through CRISPRi strategy, and the expression levels of TIC marker were examined by realtime PCR. b LncGPR107 depleted and control cells were used for sphere formation detection. Sphere photos and sphere formation ratios were shown in left and right panels, respectively. c 10, 1 × 10², 1 × 10³, 1 × 10⁴ and 1 × 10⁵ lncGPR107 depleted cells were injected into BALB/c nude mice. Three month later, tumor-free mice were observed and liver TIC ratios were calculated through ELDA analysis (lower panels). n = 6 for each group. d 3 × 10⁵ lncGPR107 depleted cells were seeded onto Matrigel-coated membrane, and tumor invasion was observed 36 h later. e, f HCC primary cells were treated with lncGPR107 antisense oligo (ASO), followed by sphere formation (e) and tumor invasion (f) detection. g LncGPR107 was activated in primary cells through CRISPRa strategy, followed by realtime PCR detection for indicated genes. h LncGPR107 activated and control cell were used for sphere formation assay. Representative images and sphere-initiating ratios were shown. i 10, 1 × 10², 1 × 10³, 1 × 10⁴ and 1 × 10⁵ lncGPR107 activated cells were injected into nude mice for 3 months’ tumor initiation, and tumor-free ratios were shown. n = 6 for each group. Experiments were repeated four times