Figure 5.

Vesicle release regulated by SOCE, Ral, and the exocyst in pupal neurons regulates flight in adults. A, Cartoon representation of a pupal neuron where SOCE regulates Ral expression. Ral is required for tagging secretory vesicles toward the exocyst complex, thus marking them for both somatic and synaptic release. Such regulated vesicular release of dopamine from pupal neurons appears essential for maturation of a neural circuit required in adults for maintaining the duration of flight bouts. B, In conditions of dStim knockdown, when SOCE is attenuated, Ral levels are reduced, and this in turn has a negative impact on vesicular release in dopaminergic neurons. Consequently, flight bout durations in adult flies are also reduced.