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. 2018 Jun 20;20:61. doi: 10.1186/s13058-018-0987-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Effect of 17β-oestradiol and EGF on cell proliferation and induction of MAPK protein expression in breast cancer cells. (c) SKBR3 breast cancer cells were treated with 5, 10 and 50 ng/ml EGF and 17β-oestradiol (10^− 8 mol/l) alone and in combination for 10 min. 40 μg protein was electrophoresed on a 10% gel and transferred to nitrocellulose. The membrane was probed with rabbit anti-Phospho-Erk1/2 antibody (Thr202/Tyr204 - Cell Signalling # 4370) and mouse anti-Erk1/2 antibody (Cell Signalling # 4696)