Fig. 2.

17β-oestradiol and EGF induced cell proliferation and raf phosporylation is mediated through EGFR. (b) SKBR3 and MCF-7 breast cancer cells were pre-treated with the EGFR antagonist AG1478 (150 nmol/l) for 1 h before 10 min of incubation with EGF (10 ng/ml) and 17β-oestradiol (10^− 8 mol/l) alone and in combination. 40 μg protein was electrophoresed on a 10% gel and transferred to nitrocellulose. The membrane was probed with rabbit anti-Phospho-Raf antibody (Ser259 - Cell Signalling # 9421) and mouse anti-Raf antibody (Santa Cruz sc-373,722)