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. 2018 Jun 20;20:61. doi: 10.1186/s13058-018-0987-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The role of the AT1 receptor in 17β-oestradiol and EGF mediated cell proliferation and raf phosphorylation breast cancer cells. (b) SKBR3 cells were pre-treated with the AT1 antagonist saralasin (10^− 6 mol/l) for 1 h before 10 min of incubation with EGF (10 ng/ml) and 17β-oestradiol (10^− 8 mol/l) alone and in combination. 40 μg protein was electrophoresed on a 10% gel and transferred to nitrocellulose. The membrane was probed with rabbit anti-Phospho-Raf antibody (Ser259 - Cell Signalling # 9421) and mouse anti-Raf antibody (Santa Cruz sc-373,722)