Fig. 7.

Validation of the expression patterns of twelve known and four novel differentially expressed miRNA-target modules by stem-loop qRT-PCR and qRT-PCR. qRT-PCR-based and stem-loop RT-PCR-based results showing a combined histogram of the inverse expression between miRNAs and their potential targets in E7 under salt stress for 4 h (a) and 24 h (b). miRNA expression (top) was validated by sequencing small RNAs isolated from control (ck) and salt-treated (4 h and 24 h) samples. The miRNA targets (bottom) were validated by mRNA-seq, and their expression levels were checked by qRT-PCR. Up- or down-regulation in expression was normalized to that of U6 snRNA or actin. The black triangle indicates that the conserved interactions of the sequenced miRNA targets were validated by the published degradome sequencing data using PAREsnip