K:Molv NIH 3T3 cells were incubated with 10 μM BGlc4 for 24 h, rinsed three times with PBS buffer, and fixed with 4% paraformaldehyde solution. Confocal microscope excitation at 514 nm, emission monitored with a 710–750 band-pass filter. Under similar conditions using the IGlc4, CGlc4, or PGlc4, no fluorescence images are observed using a 610–650 nm emission band-pass filter. The image is not enhanced; magnification is 60×. Reproduced with permission from ref 234. Copyright 2010 American Chemical Society.