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. Author manuscript; available in PMC: 2018 Jun 21.
Published in final edited form as: Curr Eye Res. 2016 Jul 21;42(4):520–527. doi: 10.1080/02713683.2016.1200099

Table 2.

Relative expression of the 35 genes by corneal keratocytes in central and peripheral stroma.

Gene name Gene description Central stroma
Peripheral stroma
Mean CT Mean R Mean CT Mean R
COL6A2 Collagen, type VI, alpha2 26.44 11.50 29.63   3.51
SPARC* Secreted protein, acidic, cysteine-rich 26.85 10.73 31.29   1.37
COL12A1 Collagen, type XII, alpha 1 26.08   7.46 29.38   3.69
TGFBI* Transforming growth factor, beta-induced, 68 kDa 26.17   7.09 28.76   3.48
TIMP2 TIMP metallopeptidase inhibitor 2 26.22   5.79 30.07   1.33
ECM1 Extracellular matrix protein1 27.16   3.47 31.33   0.75
TIMP1* TIMP metallopeptidase inhibitor 1 27.78   3.24 32.43   0.56
MMP2* Matrix metallopeptidase 2 27.19   2.53 32.17   0.53
FN1 Fibronectin 1 29.56   2.11 29.27   2.64
SPG7 Spastic paraplegia 7 29.81   1.08 33.58   0.09
LAMA3 Laminin, alpha 3 30.90   1.03 34.56   0.13
COL1A1* Collagen, type I, alpha1 32.78   0.87 32.21   0.78
ITGB4 Integrin, beta 4 29.43   0.76 30.41   0.83
CD44 CD44 molecule 30.25   0.70 31.86   0.35
ITGB5 Integrin, beta5 29.82   0.53 32.94   0.22
CTNND1 Catenin, delta 1 30.52   0.45 33.24   0.20
THBS1* Thrombospondin 1 29.90   0.41 30.69   0.82
CTNNB1 Catenin, beta 1, 88 kD 31.03   0.40 32.99   0.21
VTN Vitronectin 30.65   0.34 33.33   0.22
ITGB1* Integrin, beta 1 29.08   0.32 30.72   0.35
CTNNA1 Catenin, alpha 1, 102 kD 30.94   0.30 31.28   0.25
CTGF Connective tissue growth factor 30.48   0.28 32.08   0.36
COL7A1 Collagen, type VII, alpha1 30.00   0.24 33.75   0.09
COL8A1 Collagen, type VIII, alpha1 33.95   0.24 33.12   0.14
ITGA5 Integrin, alpha 5 33.21   0.20 33.66   0.05
ADAMTS1 ADAM metallopeptidase with thrombospondin type 1 34.39   0.19 33.36   0.36
THBS2* Thrombospondin 2 29.93   0.17 33.53   0.12
ITGA6 Integrin, alpha 6 27.78   0.16 28.55 13.43
MMP3 Matrix metallopeptidase 3 31.24   0.11 31.30   0.71
THBS3 Thrombospondin 3 31.53   0.10 34.57   0.03
ICAM1* Intercellular adhesion molecule 1 31.08   0.07 32.88   0.50
ITGA1 Integrin, alpha 1 31.28   0.07 33.73   0.07
COL5A1* Collagen, type V, alpha1 33.16   0.07 32.17   3.04
MMP14* Matrix metallopeptidase 14 33.04   0.06 33.58   0.04
ITGA4 Integrin, alpha 4 34.54   0.03 36.11   0.02

The housekeeping gene GAPDH served as control for normalization.

CT: threshold cycle; RE: Relative expression.

*

Genes previously known to be expressed in the corneal keratocytes with specific functions and involvement in stromal diseases. Other genes listed were not previously described in keratocytes. The six most abundant genes in central stroma are marked in bold; the six most abundant genes in peripheral stroma are shown in shade or kept in bold.