Table 1.
Analysis of H-DNA-Induced and Spontaneous (B-DNA) Mutations in Human Cells
| Cells | Replication Status | H-DNA | B-DNA Control | ||
|---|---|---|---|---|---|
|
|
|
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| Deletions | Point Mutations | Deletions | Point Mutations | ||
| XPF+ | + | 77.8% (28/36) | 22.2% (8/36) | 38.9% (7/18) | 61.1% (11/18) |
|
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| XPF− | + | 41.4%a (12/29) | 58.6% (17/29) | 53.8% (14/26) | 46.2% (12/26) |
|
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| XPG+ | + | 71.4% (15/21) | 28.6% (6/21) | 41.2% (7/17) | 58.8% (10/17) |
|
| |||||
| XPG− | + | 33.3%b (7/21) | 66.7% (14/21) | 12.5% (2/16) | 87.5% (14/16) |
|
| |||||
| XPA+ | + | 71.4% (10/14) | 28.6% (4/14) | 11.1% (2/18) | 88.9% (16/18) |
|
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| XPA− | + | 56.5% (13/23) | 43.5% (10/23) | 26.7% (4/15) | 73.3% (11/15) |
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| HeLa, siCON | + | 77.8% (14/18) | 22.2% (4/18) | 66.7% (6/9) | 33.3% (3/9) |
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| HeLa, siFEN | + | 100.0%a (43/43) | 0.0% (0/43) | 92.9% (13/14) | 7.1% (1/14) |
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| XPF+, siCON | − | 15.0% (3/20) | 85.0% (17/20) | 0.0% (0/21) | 100.0% (21/21) |
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| XPF+, siCON | + | 47.4% (9/19) | 52.6% (10/19) | 4.4% (1/23) | 95.6% (22/23) |
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| XPF−, siCON | − | 12.5% (1/8) | 87.5% (7/8) | 19.0% (4/21) | 81.0% (17/21) |
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| XPF−, siCON | + | 34.8% (8/23) | 65.2% (15/23) | 0.0% (0/19) | 100.0% (19/19) |
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| XPF+, siFEN | − | 30.8% (4/13) | 69.2% (9/13) | 25.0% (2/18) | 75.0% (16/18) |
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| XPF+, siFEN | + | 28.0% (7/25) | 72.0% (18/25) | 6.2% (1/16) | 93.8% (17/16) |
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| XPF−, siFEN | − | 14.3% (1/7) | 85.7% (6/7) | 83.3% (10/12) | 16.7% (2/12) |
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| XPF−, siFEN | + | 15.8% (3/19) | 84.2% (16/19) | 5.3% (1/19) | 94.7% (18/19) |
a
p value < 0.01 in χ2 test
b
p value < 0.05