Table 2.
Glycosyl hydrolase (GH) families relevant for anaerobic digestion according to Vanwonterghem et al. (2016) and their respective transcript per million (TPM) values and transcriptional categories for each of the four analysed genome bins. The categories range from 0 (no transcription) and 1 (lowest 10% of transcripts) to 10 (top 10% transcripts). n.d.: not detected
| Enzymes | Glycoside | Thermotogae bin | Fusobacteria bin | Spirochaetes bin | Cloacimonetes bin | ||||
|---|---|---|---|---|---|---|---|---|---|
| Enzyme type | Hydrolase family | TPM | Category | TPM | Category | TPM | Category | TPM | Category |
| Endo – and exo‐1,4‐β‐D‐glucanase (cellulase) | GH5 | 3.639 | 3 | n.d. | – | n.d. | – | n.d. | – |
| Hemicellulose | GH16 | 4.340 | 3 | n.d. | – | n.d. | – | 0 | 0 |
| GH28 | 2.167 | 2 | n.d. | – | 0.023 | 2 | n.d. | – | |
| GH53 | 41.236 | 8 | n.d. | – | n.d. | – | n.d. | – | |
| GH115 | n.d. | – | n.d. | – | 0.132 | 6 | n.d. | – | |
| GH76 | n.d | – | n.d | – | 0.018 | 1 | n.d | – | |
| Starch and glycogen Hydrolase | GH13 | 3.958 | 3 | 1.574 | 6 | 0.129 | 6 | 0.007 | 2 |
| GH77 | n.d. | – | 9.716 | 10 | 0.102 | 6 | 0 | 0 | |
| GH57 | 35.201 | 8 | 3.788 | 8 | 0.165 | 7 | 0.021 | 5 | |
| Lysozyme, chitinase (cell wall degradation) | GH18 | 6.172 | 3 | n.d. | – | n.d. | – | 0.021 | 5 |
| GH23 | 20.029 | 6 | 0.655 | 3 | n.d. | – | 0.063 | 8 | |
| GH73 | n.d. | – | n.d | – | n.d | – | n.d | – | |
| Glycosidase (hydrolysis of single sugar residues from non–reducing ends) | GH1 | 0.822 | 1 | n.d. | – | 0.074 | 4 | n.d. | – |
| GH2 | 38.604 | 8 | n.d. | – | 0.107 | 6 | n.d. | – | |
| GH3 | 3.022 | 2 | n.d. | – | 0.142 | 6 | n.d. | – | |
| 0.069 | 4 | ||||||||
| GH4 | 22.249 | 6 | n.d. | – | 0.019 | 2 | n.d. | – | |
| 144.144 | 10 | ||||||||
| GH38 | n.d. | – | n.d. | – | n.d. | – | 0.004 | 1 | |
| GH51 | 3.355 | 3 | n.d | – | 0.113 | 6 | n.d | – | |
| Oligosaccharide phosphorylase | GH130 | n.d. | – | n.d. | – | n.d. | – | 0.002 | 1 |