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Identification of the recombinant Vibrio alginolyticus strain 16‐3 harboring plasmid pBV220‐lysisE by PCR and restriction enzyme digestion with EcoR I and Pst I. Lane M1: Marker DL2000, Lane1: PCR product of lysis E gene, Lane 2: Temperature‐controlled lysis plasmid pBV220‐lysisE double‐digested with EcoR I and Pst I, Lane 3: Temperature‐controlled lysis plasmid pBV220‐lysis E, Lane M2: Marker DL10000
