Figure 4.

The expression of ropAe gene is upregulated under copper deficiency. The transcriptional responses of actP and ropAe to copper excess (PY + 0.5 mM CuCl₂) and copper deficiency (PY+ 2mmol/L chelating agent BCDS) were analyzed by qPCR. The copper excess panel shows a 25‐fold increase in the mRNA level of actP when wt R. etli was exposed for 30 min to an overload of 0.5 mmol/L CuCl₂. The copper deficiency panel shows a 17‐fold increase in the mRNA level of ropAe when R. etli was culturedovernight in the presence of 2 mmol/L of the Cu⁺/Cu²⁺chelating agent bathocuproine disulfonic acid (BCDS). Both assays were normalized to hisCd mRNA level (mean ± SD., n = 3) (t test, **p ≤ .05)