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The translocon components EspA, EspB, and EspD are dispensable for calcium sensing. The secretion profiles of the EPEC wild‐type strain (WT) and the ∆espA, ∆espB, and ∆espD null mutants grown under T3S inducing conditions in the presence or absence of calcium were analyzed by Coomassie brilliant blue stained SDS‐PAGE (upper panel). Immunodetection of secreted proteins (SN) and whole‐cell lysates (WC) was performed using specific antibodies against Tir and DnaK (bottom panel)
