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. 2018 Jun 22;41(6):274–283. doi: 10.1097/CJI.0000000000000229

FIGURE 3.

FIGURE 3

IL-21 enhances proliferation and prevents apoptosis of activated naive CD8+ T cells. A, Cell counting of CD8+ T cells in day 0, 5, 10, and 15 with standard medical blood count board. B, Total CD8+ TSCM-cell numbers from day 0 to day 14. Cell number was calculated as the total CD8+ T-cell number multiplied by the percentage of CD8+ TSCM cells accessed by flow cytometric analysis in the indicated time points. C, The number of CD8+ T cells stimulated with gradient doses of IL-21. D, The percentage of CD8+ TSCM cells in the indicated dose of IL-21. E, Flow cytometric analysis of CD8+ TSCM cells in the indicated dose of IL-21. Graphs showed T cells after gating on CD8+ CD45RA+ CD45RO− CD62L+ CCR7+ cells. F, CFSE-labeled naive CD8+ T cells primed with 10 ng/mL IL-2 or 20 ng/mL IL-21. Flow cytometric analysis was performed 3 days or 7 days after activation. Data were analyzed with linear regression model. IL-21 indicates interleukin-21; TSCM, T memory stem cell.