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. 2018 Jun 22;41(6):274–283. doi: 10.1097/CJI.0000000000000229

FIGURE 4.

FIGURE 4

IL-21 only highly upregulates the phosphorylation of STAT3 in the process of TSCM priming. Naive CD8+ T cells display identical STAT phosphorylation profiles in response to IL-2 or IL-21. FACS-purified CD8+ T cells from healthy donors were activated with coated anti-CD3(2 μg/mL) and soluble anti-CD28(1 μg/mL) overnight, then stimulated with 10 ng/mL IL-2 or 20 ng/mL IL-21. Two hours after stimulation, cells were lysed in sodium dodecyl sulfate polyacrylamide gel electrophoresis sample buffer. Equivalent amounts of proteins were analyzed by Western blot for indicated phosphoproteins. Data in STAT1 (A) STAT3 (B) STAT5 (C) Akt (D) and Erk1/2 (E) are shown representative of 3 experiments. IL-21 indicates interleukin-21; STAT, signal transducer and activator of transcription; TSCM, T memory stem cell.