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Construction of pALH251 for complementation of inactivated rpoN with constitutively expressed rpoS. (a) A 965-bp fragment was excised from pJRS525 and a 1,158-bp fragment containing a PflaB-kan promoter fusion was inserted into the AlwNI and FspI sites, yielding pALH227. (b) pALH251 was obtained on ligating a PflgB-rpoS fusion construct (1,224 bp) into the BamHI and NcoI sites of the multiple cloning site of pALH227.
