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. 2001 Oct 23;98(22):12724–12729. doi: 10.1073/pnas.231442498

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Copyright © 2001, The National Academy of Sciences

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PCR analysis of rpoN and rpoS mutants. (a) Schematic of PCR primer pairs (short arrows). (b and c) Agarose gel patterns of amplicons for wild type (lanes 2–4), (b) a rpoN mutant, or (c) a rpoS mutant (lanes 5–7). Lanes 1 contain DNA markers of ΦX174/HaeIII. Gene disruption by ermC results in an increased size of the amplicons (compare lanes 2 and 5). A combination of ermC-specific and flanking primers yielded only products for the mutants (lanes 6 and 7), but not for 297 (lanes 3 and 4). Lanes 3 and 6: priAH102 (arrow 102) in combination with (b) priAH59 (arrow 59) or (c) priAH27 (arrow 27). Lanes 4 and 7: priAH104 (arrow 104) in combination with (b) priAH60 (arrow 60) or (c) priAH131 (arrow 131).