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. 2001 Oct 23;98(22):12724–12729. doi: 10.1073/pnas.231442498

Figure 4.

Figure 4

RT-PCR analysis of a rpoN mutant. Borreliae were cultivated in BSK-H at pH 6.8. (a and c) Comparative RT-PCR; 10-fold dilutions of RNA from the wild type (strain 297) or the mutant were tested for rpoN (a) and rpoS (c) transcripts. Buf, buffer only; −RT, lacking RT. (b and d) Quantitative competitive RT-PCR; competitor RNA (cRNA) was used at exponential dilution, as indicated above the panels. The expression of constitutive flaB was unaffected by the rpoN mutation (b), whereas the expression of ospC was abolished (d).