Figure 5.
SDS/PAGE (Coomassie blue stain) (a) and immunoblots (b–g) of whole cell lysates (39) from wild-type, mutant, and complemented strains of 297. Total protein from about 2 × 107 spirochetes (late-logarithmic phase of growth) was loaded per gel lane, except when probing for RpoS (10-fold more material). Monoclonal antibodies against OspA and FlaB and polyclonal antisera against all other proteins have been described (23, 51). Cultivation pH and temperature are indicated above each lane. Lanes 1 and 2, wild-type 297. Lane 3, rpoN mutant. Lanes 4–6, rpoN mutant complemented with wild-type rpoN. Lane 7, rpoN mutant complemented with pALH251 (rpoS). Lane 8, rpoS mutant.