Table 1.
Recombinant plasmids
| Plasmid | Description | Source |
|---|---|---|
| pGEM-T | TA cloning vector, high copy number, Ampr | Promega |
| pCR2.1 | TA cloning vector, high copy number, Ampr, Kanr | Invitrogen |
| pUC4K | Aminoglycoside 3′-phosphotransferase gene from Tn903 (kan), Ampr, Kanr | Amersham Pharmacia |
| pJRS233 | Ermr | K. McIver (Univ. of Texas Southwestern) |
| pJRS525 | Specr, wide host range cloning vector | K. McIver (Univ. of Texas Southwestern) |
| pFAC | pGEM-T, for SP6 transcription of ospC- and flaB-specific competitor RNA | This study |
| pALH133 | pGEM-T, ermC from pJRS233, Ampr, Ermr | This study |
| pALH227 | pJRS525, Δ[spec, AlwNI-FspI(965 bp)] Ω[PflaB-kan, FspI–AlwNI (1,158 bp)], Kanr | This study |
| pALH251 | pALH227Ω[PflgB-rpoS, BamHI–NcoI (1,224 bp)], Kanr | This study |
| pALH293 | pALH227Ω[PrpoN-rpoN, BamHI–NcoI (1,628 bp)], Kanr | This study |
| pALH362 | pGEM-T, 4,631-bp fragment (analogous B31 chromosome 810459–815090), Ampr | This study |
| pALH364 | pGEM-T, 5,156-bp fragment (analogous B31 chromosome 468016–473172), Ampr | This study |
| pALH386 | pALH362, except rpoS∷ermC [BbsI (1 kb)], Ampr, Ermr | This study |
| pALH394 | pALH364, except rpoN∷ermC [AflII (1 kb)], Ampr, Ermr | This study |
| pALH400 | pALH394, except ermC∷PrpoN-rpoN-PflaB-kan [BclI (3 kb)], Ampr, Erms, Kanr | This study |