Figure 3. Effects of neutrophil depletion on innate lymphoid cell recruitment and biology.

(A–D) Neutrophils of naive, I/NT, and I/T mice were depleted, or not, as indicated in Figure 1A, and ILC in the spleen were assayed 14 days p.i. by flow cytometry. (A) Frequency of CD3^–NKp46⁺ cells in the CD45.2⁺ leukocytic population. (B) Frequency of CD117⁺/CD127⁺ cells in the Lin^–NKp46⁺ population. (C) Maturation (CD11b⁺ cells) and (D) expression of IFN-γ in the CD3^–NKp46⁺ population. (E) In vivo cytolysis activity of 667 mAb in infected/treated mice after depletion of neutrophils. The 1A8, or the 2A3 isotype control mAb, was administered to I/T mice, and 667 ADCC activity was quantified at 30 days p.i., as in Figure 2, C and D. The data represent at least 2 independent experiments. Data are expressed as mean ± SEM. Statistical significance was established using a parametric 1-way ANOVA test with a Bonferroni correction (*P < 0.05; **P < 0.01; ***P < 0.001).