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. 2018 May 3;3(9):e97339. doi: 10.1172/jci.insight.97339

Figure 4. Assay of FrCasE-specific CD8+ T cells in the presence and absence of neutrophils.

Figure 4

(A) Neutrophils of naive, I/NT, and I/T mice were depleted, or not, as indicated in Figure 1A. Frequency of FrCasE-specific CD8+ T cells. Spleen cells were isolated at day 14 p.i., and the frequency of virus-specific CD8+ T cells in the total CD8+ T cells population was assayed by flow cytometry using the H2Db-GagL MHC tetramer. The data represent 4 independent experiments, with at least 11 mice per group. (B) Expression of IFN-γ by CD8+ T cells. Splenic CD8+ T cells were analyzed by flow cytometry for the expression of IFN-γ. The data presented represent 3 independent experiments, with at least 7 mice per group. Data are expressed as mean ± SEM. Statistical significance was established using a parametric 1-way ANOVA test with a Bonferroni correction (*P < 0.05).