Skip to main content
. 2018 May 3;3(9):e97339. doi: 10.1172/jci.insight.97339

Figure 5. Enhancement of the humoral antiviral response by neutrophils.

Figure 5

Neutrophils of naive, I/NT, and I/T mice were depleted, or not, as indicated in Figure 1A. (A and B) Serum concentration of FrCasE-specific Igs. (A) Seric FrCasE-specific IgM levels were assayed by ELISA at 14 days p.i. The data represent 2 independent experiments, with 8–11 mice per group (for I/NT and I/T mice) and 3–6 mice per group (for naive mice). (B) Seric FrCasE-specific IgG concentration was assayed by ELISA at the indicated times. The data represent 2 independent experiments, with 7–9 mice per group. Data are expressed as mean ± SEM. Statistical significance was established using a parametric 1-way ANOVA test, with a Bonferroni correction. (C) Correlation between serum anti-FrCasE IgG levels (evaluated as AUC) and survival times, analyzed using the Pearson correlation test. AUC was evaluated until the last time point at which all mice were still alive (day 68 p.i.). All infected/nontreated (n = 8) and infected/treated (n = 9) mice, depleted or not of neutrophils (n = 9 and n = 7, respectively), showed in Figure 1D were evaluated for such a correlation. (D) FrCasE-specific secondary humoral response. Seric FrCasE-specific IgG levels in I/T mice (depleted or not of neutrophils) were assayed by ELISA before and 1 week after a viral challenge performed at day 93 p.i. The data represent 2 independent experiments, with 5 mice per group. Statistical significance was established using a paired Student’s t test (*P < 0.05; **P < 0.01; ***P < 0.001).