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. 2018 May 17;3(10):e98204. doi: 10.1172/jci.insight.98204

Figure 4. Synthetic IIM microRNAs induce pDC maturation and promote pDC survival.

Figure 4

(A and B) pDCs were stimulated with the indicated microRNAs (10 μg/ml) or with vehicle alone (–) for 24 hours. The surface expression of CD86 (A) and BDCA-2 (B) was evaluated by FACS analysis. Data are expressed as the mean ± SEM of the percentage of positive cells (left y axis), as well as the mean ± SEM of the median fluorescence intensity (MFI) of positive cells (right y axis) normalized over unstained controls (n = 3); *P < 0.05 versus (–) by 1-way ANOVA with Dunnett’s post-hoc test. (C) pDCs were stimulated as indicated in culture medium devoid of IL-3 for 24 hours. Cell viability was evaluated by propidium iodide staining (mean SEM; n = 3); *P < 0.05 versus (–) by paired Student’s t test. (D) pDCs were stimulated with the indicated microRNAs (10 μg/ml) in the presence or absence of IL-3 for 24 hours. IFN-α production was evaluated by ELISA. Data are expressed as mean ± SEM (n = 3); *P < 0.05 versus (–) by paired Student’s t test. (E) pDCs were stimulated as indicated or lysed immediately after purification (fresh). The expression of BCL-xL, A1, and β-actin were determined by Western blot. One representative fluorogram out of 3 performed is shown.