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. 2018 May 17;3(10):e120365. doi: 10.1172/jci.insight.120365

Figure 4. PBI-4050 treatment decreased mediators of renal fibrosis.

Figure 4

(A) Immunostaining indicates that PBI-4050 treatment from 8–20 weeks of age reduced renal expression of profibrotic and fibrotic components including collagen I, collagen IV, α-SMA (marker of myofibroblasts), and connective tissue growth factor (CTGF) in eNOS–/– db/db mice. Original magnification, ×160 in all. (B) PBI-4050 treatment inhibited renal expression levels of phospho-Smad3, an indicator of activation of the TGF-β signaling pathway in eNOS–/– db/db mice. ***P < 0.001 vs. vehicle; n = 8 in each group. Original magnification, ×250. All values are shown as mean ± SEM. P value was calculated by Student’s t test.