Figure 1. CyTOF analysis of leukocytes from bronchoalveolar lavage (BAL) fluid identifies common populations in an unsupervised fashion.

(A) Biaxial gating of intact, singlet, live, and CD45⁺ cells from 16 alveolar samples analyzed by CyTOF identifies common alveolar leukocyte populations. (B) Concatenated viSNE plot generated from the same data represented in A. The color of each dot represents its immune cell subset, as designated by manual gating shown in A. (C) Concatenated viSNE plot of all CD33⁺CD71⁺CD163⁺ AMs colored per the clinical population from which each sample was procured. AM, alveolar macrophage; IgE, IgE-expressing cells (eosinophil, basophil, and mast cells); MONO, monocyte; PMN, neutrophil.