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. 2018 May 17;3(10):e99281. doi: 10.1172/jci.insight.99281

Figure 4. CD169 and PD-L1 distinguish alveolar macrophages (AMs) procured from different clinical populations.

Figure 4

(A) The hierarchical gating strategy used to identify AMs is based on the Divisive Marker Tree (DMT) shown in Figure 2. (B) Contour plot demonstrating the manually drawn gates for CD169 and PD-L1 AM subtypes within all CD45RAloCD15loCD33hi events from all 16 alveolar samples in the HMC-BAL cohort. (C) The population frequency of each CD169/PD-L1 AM subtype was different between nonmechanically ventilated healthy subjects and mechanically ventilated critically ill subjects (n = 4 not mechanically ventilated, n = 12 mechanically ventilated). (D) The population frequency of each CD169/PD-L1 AM subtype suggested a difference between non-ARDS and ARDS subjects (n = 6 non-ARDS, n = 6 ARDS). Shown are the individual values and median ± interquartile range (IQR) population frequencies of each AM subtype among total CD45RAloCD15loCD33hi AMs. Each comparison was made with a Mann-Whitney U test. **P ≤ 0.01.