Table 1. ZIKV infection, dissemination, and transmission rates in California Aedes and Culex mosquitoes 14 and 21 days post ingestion of blood from viremic mice.
| Mosquito Species | Source in California | Generation | ZIKV strain | Blood titer log10 PFU/mL | Infected (%) | Disseminated (%) | Transmitted (%) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Day 14 | Day 21 | Day 14 | Day 21 | Day 14 | Day 21 | |||||
| Cx. tarsalis | Kern County | Colony | PR15 | 5.7, 6.4, 5.4 | 2/46 (4) | 6/20 (30) | 2/46 (4) | 1/20 (5) | 0/46 (0) | 0/20 (0) |
| Cx. quinquefasciatus | Los Angeles | F5 | PR15 | 4.6 | 0/42 (0) | 0/37 (0) | 0/42 (0) | 0/37 (0) | 0/42 (0) | 0/37 (0) |
| Ae. aegypti | Los Angeles | F6 | MA66 | 4.3, 4.8 | 73/85 (86) | 22/23 (96) | 69/85 (79) | 21/23 (91) | 45/85 (53) | 20/23 (87) |
| Ae. aegypti | Los Angeles | F6 | PR15 | 5.7, 6.4, 5.4 | 39/46 (85) | 22/23 (96) | 36/46 (78) | 18/23 (78) | 30/46 (65) | 17/23 (74) |
| Ae. aegypti | Los Angeles | F6 | BR15 | 4.7 | 18/20* (90) | n.c. | 18/20*(90) | n.c. | 15/20 *(75) | n.c. |
Infection, dissemination, and transmission rates in mosquitoes that ingested ZIKV from viremic mice, determined by ZIKV RNA detection in bodies, legs+wings, and saliva, respectively. Denominators in rates represent all mosquitoes in cohorts. Multiple values in the mouse blood titer column show viremias for individual mice just before mosquitoes were presented to feed; cohorts of mosquitoes that fed on different mice within this range of viremias were combined since preliminary analysis of each cohort showed no differences in infection, dissemination and transmission rates (data not shown). n.c. indicates samples were not collected at that time point.
*Ae. aegypti that ingested BR15 were harvested 15 dpf.