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. Author manuscript; available in PMC: 2018 Jun 21.
Published in final edited form as: J Phys Chem B. 2017 Apr 14;121(16):3925–3932. doi: 10.1021/acs.jpcb.7b00324

Figure 1. S. aureus treated with disaccharide-modified glycopeptide antibiotics at sub-MICs do not readily induce ATP leakage.

Figure 1

a, Chemical structures of disaccharide-modified glycopeptide antibiotics with increasing aliphatic side chain lengths (from left to right): vancomycin (no modification), LY309687 (trifluoromethoxybenzyl side chain), oritavancin (chlorophenyl-benzyl side chain), FNCE (N-9-fluorononyl side chain), and FBBCE (N-9-fluorobiphenylbenzyl side chain). b, ATP leakage was attempted in S. aureus harvested at OD660nm 1.5 by addition of glycopeptide antibiotics to final concentrations of 0, 1, 2, 5, 10, 50, and 100 μg/mL, and daptomycin (D) at 100 μg/mL. All glycopeptide antibiotics did not induce appreciable ATP leakage attributable to membrane depolarization at the concentrations tested. In comparison, daptomycin induced ATP leakage consistent with the membrane disruption. All error bars represent 95% confidence interval.