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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: J Immunol. 2018 May 14;201(1):215–229. doi: 10.4049/jimmunol.1700967

Figure 7. Expression of genes encoding regulators of endothelial cells and IESCs.

Figure 7

RNA was isolated from populations of cells that had been purified by FACS sorting from the small intestine of wild-type mice. cDNA was prepared and analyzed by QPCR. (A) Expression of eight genes encoding endothelial cell regulators by endothelial cells (CD45-CD31+), leukocytes (CD45+), intestinal mesenchymal cells (iMCs; CD45-GP38+CD31-) and epithelial cells (CD45-EPCAM+). Mean expression in iMCs is set to 1. *p< 0.05, **p< 0.01, ***p< 0.001; one way ANOVA. Only significant differences between iMCs and other cell types are shown. (B) Expression of genes encoding key IESC regulators by Ackr4- and Ackr4+ cells purified from the CD34+ iMC population (CD45-GP38+CD31- cells). Mean expression in CD34+Ackr4+ iMCs is set to 1. ***p< 0.001; Student’s t test. Gene names are indicated at the top of each graph. Data points from individual mice are shown, along with means (±1SD).