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. Author manuscript; available in PMC: 2018 Jun 21.
Published in final edited form as: Oncogene. 2018 Mar 7;37(24):3216–3228. doi: 10.1038/s41388-018-0126-2

Figure 1. Transcriptional acivity of CEBPA51 in vitro.

Figure 1

(A) CEBPA-51 saRNA transfection increases CEBPAmRNA in murine AML12, Rattus (Clone 9), Human (HEPG2) and human (Primary hepatocytes). Key factors for liver function were screened in CEBPA-51 transfected primary human hepatocytes. These included (B) albumin, HNF4A (Hepatocyte nuclear factor 4-alpha) and CYP3A4 (Cytochrome P450 3A4). Data is expressed as mean ±SEM. ¥=p(<0.00027) and *= p(0.0033). (C) A Western blot panel probed with antibody specific to HNF4A (Abcam; ab41898), CYP3A4 (Abcam; ab155029), Albumin (Abcam; ab106582) and CEBPA (CELL Signalling; 8178) showed visible increase in protein expression in cells transfected with CEBPA51. Actin expression (Abcam: ab8226) was used as protein loading control across each of the lanes.