Table 1.
Primer sequences and targets for MAP respiratory virus array.
| Organism | Molecular target | Product size (bpa) | Primer name | Sequence |
|---|---|---|---|---|
| Influenza A | M1 | 110 | VIR13095F | TCAGGCUCCCUCAAAGCCGA |
| VIR13096R | /5MAXNb/TGCAGGATTGGTCTTGTCTTTAICCA | |||
| Influenza A | PA | 74 | VIR13099F | CTUGAGAAUTTUAGAGCCUATGUIGAUGG |
| VIR13100R | /5MAXN/CACATTTGAGAAAGCTTGCCCTCAAT | |||
| Influenza B | PB1 | 83 | VIR13470F | CAGGCAGCAAUTUCAACAACATUCCC |
| VIR13471R | /5MAXN/TTGTCTATTGTGTAGCCTGTTCCTGTTC | |||
| Influenza B | PA | 78 | VIR13491F | GGAGGGAAAAAUCUGTGUACCTGUATUGC |
| VIR13492R | /5MAXN/TAGCTTCCATTCCCCATTTCATTTGGAT | |||
| RSV | Matrix protein | 83 | VIR13361F | AAGAUGGGGCAAAUAUGGAAACAUACGUGAA |
| VIR13363R | /5MAXN/TAGAACATTGTACTGAACAGCTGCTGTGTA | |||
| VIR13362R | /5MAXN/TAGGACATTGTATTGAACAGCAGCTGTGTA | |||
| RSV | Phosphoprotein | 84 | VIR13355F | TCGGCUCGUGAUGGAAUAAGAGAUGC |
| VIR13357F | TCUGCUCGGGAUGGUATAAGAGAUGC | |||
| VIR13356R | /5MAXN/CGTCATTAATGCTTCAGTTCTGATTTTTTCTATCAT | |||
| VIR13358R | /5MAXN/CGTCATTAATGCTTCTGCTCTTATTTTTTCTATCAT | |||
| MERS | Orf1A protein | 92 | VIR13088F | CGGCCUUCAACUGGUUGUUGUU |
| VIR13089R | /5MAXN/AGCATAATTGTATGACCGCCAGTC | |||
| MERS | N protein | 102 | VIR13090F | CCUGUGUACUUCCUUCGGUACAGU |
| VIR13091R | /5MAXN/GTAGGCATCAATATTTTGCTCAAGAAGC |
Organism targets, predicted PCR product size, primer name, and primer sequences are listed here.
a
Base pairs.
b
5MAX (NHS ester).