Table 2.
Reagent concentrations for the MAP respiratory virus assay cards.
| UDG (Excipient X4)—100 μL in digestion well |
| 10 U UDG |
| 3% Trehalose |
| 1.5 mmol/L Tris |
| 7.5 mmol/L KCL |
| 0.015 mmol/L EDTA |
| 0.15 mmol/L DTT |
| 0.0015 % BSA |
| 50.00 nM hybridization control |
| Kapa (Excipient X2.2)—150 μL in PCR Master Mix chamber |
| 16 U Kapa |
| 2 % Trehalose |
| 266.67 μmol/L dNTP mixture |
| 4.67 mmol/L MgCL2 |
| 1.33 X Buffer A |
| SSIII (Excipient A)—150 μL in MM chamberc |
| 50 U SuperScript III |
| 2 % Trehalose |
| 5 mmol/L Tris |
| 1 mmol/L ammonium sulfate |
| 1.2 mmol/L MgCL2 |
| 0.005 % Tween-40 |
| 0.4 mmol/L DTT |
| 0.015 μg random primers |
| 0.03 μg Poly-A |
Composition of each of the 3 lyophilized bead reagents provided with the Map cards, and the resulting reagent concentrations or compositions following resuspension thereof in the chambers of the card in which the beads were packages are represented. Super Script III (Life Technologies, Carlsbad, CA).