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. 2018 Jun 21;13(6):e0199233. doi: 10.1371/journal.pone.0199233

Fig 7. Relative levels of islet and downstream gene transcripts in morphants vs. mutants.

Fig 7

qPCR was used to compare expression levels of islet1, islet2a and islet2b between wildtype (wt), mutant (mut), morphant (morph) and Ctl MO-injected (Ctl MO) embryos. In islet2a mutants, islet1 levels were significantly increased by ~ 25%; no changes in the levels of other islet transcripts were detected in mutants. Both morphants and Ctl MO-injected embryos had significantly decreased levels of islet1. The largest change detected was a ~ 4-fold increase in islet2a levels in morphant compared to wildtype or mutant. ***, p<0.001; **, p<0.003; *, p<0.005; ANOVA with post hoc Bonferroni. To facilitate comparisons, for each gene, expression levels were normalized to that of the control group.