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. 2018 Jun 21;13(6):e0199332. doi: 10.1371/journal.pone.0199332

Fig 2. Wnt3a increases B-1 cell proliferation in vitro.

Fig 2

Purified B-1 cells from C57BL/6 mice were were stimulated with recombinant Wnt3a protein (100ng/mL) was added daily. (A) Representative dot plots of proliferation of B-1 cells: time zero (zero), non- treated cells (NT) and in the presence of Wnt3a. After 72 hrs, percentage (B) and absolute number (C) of B-1 cells in proliferation were determined. The Wnt activation was evaluated by qPCR by Axin2 gene expression (D). Normalization was performed using Rplp0 as reference gene. Non-treated B-1 cells were used as normalizer sample. Relative expression was determined using 2-ΔΔCT. Data from a representative of 3 experiments performed at triplicate from 3 biological samples. **p≤0,001 (Mann-Whitney test).