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. 2018 Jun 21;13(6):e0199332. doi: 10.1371/journal.pone.0199332

Fig 3. IL7R overexpression in Wnt3-treated B-1 cells.

Fig 3

Purified B-1 cells from C57BL/6 mice were daily treated or not (NT) with 100 ng of Wnt3a (Wnt3a) recombinant. After 72 hrs, the relative expression of lymphoid transcription factors FLt3 (A), IL7R (B), Pax-5 (C) were calculated, using non-treated group (NT) as a normalized sample. Normalization was performed using Rplp0 as reference gene. Relative expression was determined using 2-ΔΔCT. Data from a representative of 2 experiments performed at triplicate. 3 biological samples were used in each experiment. *p≤0,01 and **p≤0,001 (Mann-Whitney test). D. MFI (Mean of Fluorescence Intensity) of IL7R expression by B-1 cells in the presence of Wnt3a or not (NT). Data from a representative of 2 experiments performed (n = 5). E. MFI (Mean of Fluorescence Intensity) of Flt3 expression by B-1 cells in the presence of Wnt3a or not (NT). Data from a representative of 2 experiments performed (n = 5).