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. 2018 Jun 21;13(6):e0199332. doi: 10.1371/journal.pone.0199332

Fig 5. Canonical Wnt signaling is activated in B-1 precursors, but not in B-1 cells.

Fig 5

B-1 progenitors from bone marrow and peritoneal B-1 cells from Axin2+/lacZ and WT mice were isolated and the canonical Wnt signaling analyzed based on β-galactosidase (FDG+) activity. The percentage (A-C) and absolute number (B-D) FDG+ of each cell population were then calculated. n = 3 mice per group. *p≤0,01 (Mann-Whitney test). WT mice not carrying the reporter transgene (Axin2+/lacZ) were used to define the FDG− population. In order to calculate the percentage or absolute number of FDG+ cells of each subset, FDG+ amount was subtracted from amount of FDG+ cells in the WT mice in order to correct differences in background staining.