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. 2018 Jun 21;13(6):e0199353. doi: 10.1371/journal.pone.0199353

Fig 3. Fab-8 blocks the propagation of Ubx PRE-mediated repressive H3K27me3 and H3K9me3 marks in homozygous transgenic embryos.

Fig 3

(A, B, C, D) The ChIP-qPCR expreiments were performed with chromatin isolated from 20–24 h transgenic embryos. PRE-RT region or Rpl32 coding region was used as a positive control or a negative control for H3K27me3 binding. (E, F, G, H) H3K9me3 ChIP-qPCR expreiments were performed with chromatin isolated from 20–24 h transgenic embryos. The heterochromatic control region F22 and the intergenic region of euchromatin F19 were used as a positive control and a negative control for H3K9me3 ChIP-qPCR, respectively. Background immunoprecipitation was subtracted from normalized specific antibody ChIP signals at each position examined.