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Colon crypts were isolated from mouse and cultured embedded with Matrigel in growth factors containing DMEM/F12 media. Two distinct morphologies of cultures can be identified, namely: (A) Colonospheres, which are spherical or cystic in shape without crypt-like projections. (B) Colonoids, which are oblate ellipsoids shaped structures with crypt-like buds extending outwards into the Matrigel.
