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. 2018 Jun 21;13(6):e0199412. doi: 10.1371/journal.pone.0199412

Fig 7. Bone morphogenetic protein 4 (Bmp4) inhibits colonoid formation.

Fig 7

Recombinant human Bmp4 (50 and 100 ng/ml) and noggin (100 ng/ml) was added to the colon crypt cultures on Day 0, 2 and 4 and assayed in duplicates. (A) Representative bright field Extended Depth of Field (EDF) images of the cultures on Day 6. (B) The numbers of colonospheres and colonoids were scored on Day 6 and the colony formation efficiencies were determined and tabulated. Error bars and analysis: Mean ± SEM, * p< 0.05, n = 3, scale bar = 100 μm, 2-way ANOVA multiple comparisons, Tukey’s test.