Fig 8. Inhibitors of TGF-β signaling enhanced colonoid formation.

(A) A83-01, inhibitor of the TGFβ receptor kinase, was assayed at 0.1, 0.3 and 0.5 μM in duplicate culture of colon crypts with cYH2CM (50% v/v). The numbers of colonospheres and colonoids were counted on Day 6 and the colonosphere and colonoid formation efficiencies were determined. (B) Representative images of cultures on day 6. Error bars and analysis: Mean ± SEM, n = 3, * p<0.05, scale bar = 100 μm. 2-way ANOVA Fisher’s LSD test.